Sindiswa Lukhele is on Facebook. Join Facebook to connect with Sindiswa Lukhele and others you may know. Facebook gives people the power to share and. Sindiswa Lukhele of University of the Witwatersrand, Johannesburg (wits) with expertise in: Cancer Research and Cell Biology. Read 2 publications, 3 questions. Abstract: Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year.
Lukhele Sindiswa T.
Seven hundred microlitres of. After 30 min, cells were cent rifuged at rpm for. The pellet was was hed twice. The cel ls were analyse d with. Protein content was measured by the BCA. SDS polyacrylamide gel and then transferred onto. Membranes w ere subse-. Santa Cruz Biot echnology, CA. The me mbranes were. Experiments were performed in duplicates. The p -value wa s analyse d in. To determine half maximal inhibitory concentration.
Cam ptothecin, as our po sitive contro l,. This was not the case in cannabi-. Dimethyl sulfoxide DMSO was included as a. Whereas ethanol exhibited between 7.
Effect of Cannabis sativa extracts and cannabidiol on. Cells were seeded in an E-plate and allowed to attach. Little can be read from xCELLig ence except that can-. Suggest ing that c annabi diol is th e most ef fective. Cannabis sativa extracts and cannabidiol induce. Flow cytometry revealed a significant increase in SiHa. MTT assay was conducted to determine IC. SiHa, HeLa, and ME cells wit h differen t doses of butanol ex tract a , b , c , hexane extra ct d , e , f , and cann abidiol ex tract g , h , i fo r a period of.
The le vel of signi ficance w as determi ned using St udents t -T est with. HeLa cells, apoptosis was increased to A similar events was observed following.
Cannabidiol was also tested for. The results further confirmed that the type of cell. Figure shows that can-. Cannabid iol was mor e effectiv e in induci ng apoptos is. In comparison to both extracts of Cannabis sativa. SiHa cells cannabidiol induced Effect of Cannabis sativa extracts and cannabidiol on the. To characterise the cell death type following treat-.
Treatment of SiHa and HeLa cells with. Another featur e that is a repres entative of ce ll death is the. Morphologic al appearanc e of. Exposure of SiHa and HeLa cells. This was done in order to determine whether. Cannabis sativa and cannabidiol deplete ATP levels in. ATP levels were first detected after 2 h. In HeL a cells treated. Whereas in ME 0. Similar results we re observed in cannabi diol treated ce lls. A prolonged incub ation period 24 h of.
As shown in Fig. Similar result s were. There was no signifi-. SiHa cells so an increase from. Cells were treated with. ME was fairly increased. Effect of Cannabis sativa extracts and cannabidiol on cell. We further assessed the effect s of Cannabis sativa ex-. Flow cytometry showed that in the pres-. In HeL a cells, butanol. Each cell line re sponded differ ently to cann abidiol treat -.
A similar event was observed duri ng treatment. Can nabidiol sign ificantly incr eased sub-. G0 in ME ce lls to From this da ta, we.
From the apoptosis experiments conducted, it is clear. However, we needed to confirm whether the type of. In butanol extract p Cells were incubated with IC. Cannabis sativa extracts for a pe riod of 24 h. BXfl uorescenc e confoca l microsco py. In all cell lines the level. Following treatment of cervical canc er cells, Bax pro-. Western blot anal ysis revealed that ca nnabidiol effecti vely. Caspases play an ef fective role in th e execution of ap op-.
In all Cannabis s ativa extracts,. Similar resul ts were also observed in cannabi diol treated. Cervical cancer remains a burden for women of Sub-. Half a million new cases of cer vical. Therefore, it is important to search for. However, some of the medicinal plants used. It is therefore important to. In the present study, cervical cancer cell lines. We first determined whether Cannabis sativa extract s. MTT assay determ ines IC. Cannabis sativa extracts were able to reduce. These results correlate with the findi ngs.
According to [7, 24, 25]. Cannabis sativa extracts rich in cannabidiol were able to. Therefore, in this study, cannabidiol. Camptothecin functions as an inhibitor. DNA strands [19, 20]. This in turn causes DNA strands to.
Upon treatment of SiHa and HeLa cells. However , at a similar IC. In comparison to butanol and hexane. Differences in the findings could be attrib-. MTT assay is an. Induction of cell death. Untreated and camptothecin were included as. Cell death can be characterized by a decrease in the.
Therefore, to evaluate the effect of treatment on. ATP acts as determinant of both cell death. Exposure of SiHa, HeLa , and. According to , a reduction of the ATP. Following confirmation that Cannabis sativa and can-. This method uses a PI. In this study, propidium. Viable cells with an intact.
Treatment of SiHa cells with butanol and. When compared to the S-pha se and G. And thus, according to , signals DNA synth esis. A decrease in the S-phase. Bar graph a and d rep resents.
This was not the. Cannabidiol resulted in the accumulation of cells in the. SiHa, and HeL a, and. ME cells were committed to the cell death phase. Apoptosis plays a major role in determining cell sur-. Since treatment caused the accumulation of. Treatment of all three cell lines with camptothecin, IC. Untreated protein was used as a control. Initiator caspase-9 and effecter caspase-3 were included to elucidate apoptosis induction.
Apoptosis is characterized by morphological changes. FITC suggests the induction of apoptosis, since it can. Apoptosis is known to occur via two pathwa ys, the. Cannabis sativa isolates including cannabidiol have. However, not much has been.
Our focus in this study was also. The genes analyzed are p53 and RBBP6. Equal amount of protein conc was loaded in each well. Note that the darker the bands increased expression of the gene. In respons e to stress stimuli. Mutation of p53, implicated to be. Bax and Bcl-2 form part of the proteins. Following activation, p53 translocates into the cytosol. An imbalance between Bax and Bcl-2 has been. It is theref ore. Camptothec in, hexane extrac t, and. Apart from Si Ha and HeLa, butanol.
Interestin g to note is that butanol extrac t reduced the. However , we came to. We further demons trated that Canna bis sativa. Caspases play an effective role in the execution of. In this study, we wanted to validate whether. We demonstrated the ability. However, execution of apoptosis was either. Western blot revealed that Cannabis.
Thi s was not the case with buta nol. Caspa se-3 was not up -modula ted in. Howeve r on the basis of the Western blot. Cannab idiol effect ively up-.
From the resu lts we can con clude that, ap optosi s. The aim of this st udy was to evaluate for the anti-growth. Cannabis sativ a extracts was compare d to that of canna-.
The study sh owed. It further demo n-. More rese arch needs to be done elucida ting the. The datasets supporting the conclusions of this article are included within. Both authors read and approved the final manuscript. In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. Apoptosis Cervical cancer Cannabidiol Cannabis sativa.
Submitting the report failed. If the error persists, contact the administrator by writing to support infona. You can change the active elements on the page buttons and links by pressing a combination of keys:. Polski English Login or register account. Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells. Lukhele , Lesetja R. Abstract Background Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year.
Results Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. Conclusions In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. Assign yourself or invite other person as author. It allow to create list of users contirbution. Assignment does not change access privileges to resource content.
You're going to remove this assignment. Additional information Copyright owner: Fields of science No field of science has been suggested yet.
Sindiswa T. Lukhele, Mpho S. Choene, and Lesetja R. Motadi. I dedicate this dissertation to my mother, Martha Lukhele and my late brother, Dennis. Sindiswa T. Lukhele, Lesetja Raymond Motadi; Published in BMC complementary and alternative medicine; DOI/s Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells. Sindiswa T. Lukhele and.